| 质粒名称: | pAG70 BDNF 3'UTR |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 12021 |
| 存储实验室: | David Bartel |
|
载体骨架基本信息 |
|
| 载体名称: | pIS2 |
| 载体出品公司: | pIS2源自pRL-SV40（Promega） |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BDNF 3'UTR,BDNF,brain-derived neurotrophic factor |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1141 bp |
| 基因突变: | -- |
| GenBank编号 | NM_170735 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacI(未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | EBV rev primer (GTGGTTTGTCCAAACTCATC) |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAG70 BDNF 3'UTR was a gift from David Bartel (Addgene plasmid # 12021 ; http://n2t.net/addgene:12021 ; RRID:Addgene_12021) |
| 参考文献部分: | The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, Bartel DP. Science. 2005 Dec 16. 310(5755):1817-21. 10.1126/science.1121158 PubMed 16308420 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAG70 BDNF 3'UTR 4866 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAG70 BDNF 3'UTR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4866)
AUTHORS Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB,
Bartel DP
TITLE The widespread impact of mammalian MicroRNAs on mRNA repression and
evolution.
JOURNAL Science. 2005 Dec 16. 310(5755):1817-21.
PUBMED 16308420
REFERENCE 2 (bases 1 to 4866)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4866
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 1181..1302
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1192..1211)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1246..1265
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 1435..1539
/gene="bla"
/label=AmpR promoter
CDS 1540..2400
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1758..1777)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2571..3159
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3060..3079
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 3288..3645
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3311..3331)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3496..3631
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3558..3577
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 3715..3847
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 3830..3849
/label=pMT2-F
/note="Synthetic intron, forward primer"
promoter 3892..3910
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3920..4855
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
1 ccagtcattt gcgcacaact taaaaagtct gcattacatt ccttgataat gttgtggttt
61 gttgccgttg ccaagaactg aaaacataaa aagttaaaaa aaataataaa ttgcatgctg
121 ctttaattgt gaattgataa taaactgtcc tctttcagaa aacagaaaaa aaacacacac
181 acacacaaca aaaatttgaa ccaaaacatt ccgtttacat tttagacagt aagtatcttc
241 gttcttgtta gtactatatc tgttttactg cttttaactt ctgatagcgt tggaattaaa
301 acaatgtcaa ggtgctgttg tcattgcttt actggcttag gggatggggg atggggggta
361 tatttttgtt tgttttgtgt ttttttttcg tttgtttgtt ttgtttttta gttcccacag
421 ggagtagaga tggggaaaga attcctacaa tatatattct ggctgataaa agatacattt
481 gtatgttgtg aagatgtttg caatatcgat cagatgacta gaaagtgaat aaaaattaag
541 gcaactgaac aaaaaaatgc tcacactcca catcccgtga tgcacctccc aggccccgct
601 cattctttgg gcgttggtca gagtaagctg cttttgacgg aaggacctat gtttgctcag
661 aacacattct ttccccccct ccccctctgg tctcctcttt gttttgtttt aaggaagaaa
721 aatcagttgc gcgttctgaa atattttacc actgctgtga acaagtgaac acattgtgtc
781 acatcatgac actcgtataa gcatggagaa cagtgatttt tttttagaac agaaaacaac
841 aaaaaataac cccaaaatga agattatttt ttatgaggag tgaacatttg ggtaaatcat
901 ggctaagctt aaaaaaaact catggtgagg cttaacaatg tcttgtaagc aaaaggtaga
961 gccctgtatc aacccagaaa cacctagatc agaacaggaa tccacattgc cagtgacatg
1021 agactgaaca gccaaatgga ggctatgtgg agttggcatt gcatttaccg gcagtgcggg
1081 aggaatttct gagtggccat cccaaggtct aggtggaggt ggggcatggt atttgagaca
1141 ttccaaaacg atctagagcg gccgcttcga gcagacatga taagatacat tgatgagttt
1201 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
1261 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
1321 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
1381 tacaaatgtg gtaaaatcga taaggatcca ggtggcactt ttcggggaaa tgtgcgcgga
1441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
1501 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
1561 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
1621 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
1681 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
1741 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
1801 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
1861 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
1921 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
1981 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
2041 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
2101 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
2161 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
2221 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
2281 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
2341 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
2401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
2461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
2521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
2581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
2641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
2701 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
2761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
2821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
2881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
2941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
3001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
3061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
3121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
3181 ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat ctgcgcagca
3241 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
3301 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct
3661 gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt
3721 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga
3781 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc
3841 tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac ttaatacgac
3901 tcactatagg ctagccacca tgacttcgaa agtttatgat ccagaacaaa ggaaacggat
3961 gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg attcatttat
4021 taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac atggtaacgc
4081 ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag cgcggtgtat
4141 tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt cttataggtt
4201 acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac caaagaagat
4261 catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct atgagcatca
4321 agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg aatcatggga
4381 tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag gagaaaaaat
4441 ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt
4501 agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg aagttcgtcg
4561 tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt
4621 acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac caaaaatgtt
4681 tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca agaagtttcc
4741 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga
4801 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataattcta
4861 ggagct
//
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